No interference with autofluorescence from membrane slidesSame unique specificity: only human sperm heads are identified.
Same sensitivity: find and identify a single spermatozoa on a slide in less than 2 minutes (smear or extract slides)
Same simple staining technique: quick fixation, sample preparation, block, stain – cover slip and visualize
Different nuclear stain: Propidium Iodide – avoids UV damage - avoids interference with PEN and PET LCM membranes
Better signal for non-coverslipped slides: brighter sperm heads
Dyes do not interfere with downstream DNA-STR analysis