HY-LITER Express
Immunofluorescence detection kit for human spermatozoa from sexual assault evidence
Two fluorescent dyes: nuclear (non-specific) + sperm (specific)
HY-LITER EXPRESS staining time reduced to ~60 minutes
- Positive identification of sperm
- Meets new audit standards for evidence preservation
- Microscopic screening of sperm
- Human specific – monoclonal antibody-based
- Fluorescent detection – debris does not interfere
Step 1: Attach cells to slide – 5 min incubation
Step 2: Sample preparation – 15 min incubation
Step 3: Blocking – 15 min incubation
Step 4: Labeling and staining – 15 min incubation
Step 5: Mounting & cover slip (optional) – 20 min
- Fast staining time: Staining time reduced to ~60 minutes
- Simple staining technique: quick fixation,
- sample preparation, block, stain – coverslip and visualize
- Unique specificity: only human sperm heads are identified
- Confirmation steps: nuclear staining with DAPI
- Sensitivity: identify a single spermatozoa on a slide in less
- than 2 minutes (smear or extract slides)
- Brighter signal than normal HY-LITER version:
- better fluorophore – brighter sperm heads
Sperm-HY-LITER EXPRESS
cat number #7302 with 50 stains per kit
SPERM HYLITER EXPRESS
sku | description |
---|---|
7301 | Small SPERM HY-LITER reagents for staining 26, 11 mm wells or 50, 8mm wells |
7302 | Medium SPERM HY-LITER reagents for staining 50, 11 mm wells or 100, 8mm wells |
7303 | Large SPERM HY-LITER reagents for staining 150, 11 mm wells or 300, 8mm wells |
7302-SOS | SPERM HY-LITER SOS – New Solution Staining Kit with International DTT, Stain 50 slides |
7303P | SPERM HY-LITER EXPRESS Large Kit w/ DTT, choice of slides and storage boxes |