Fig. 3: Dose-dependent inhibition of heparanase enzyme activity by heparin, a known inhibitor of heparanase. The calculated IC50 was 4.4 ng / ml.
Recommended Concentration Range for Inhibitors:
Potent inhibitors: 0.5 - 10 ng / ml
Less potent inhibitors: 10 - 100 ng / ml
(Based on 100 µl / well for 96-well microplates and 20 µl / well for 384 well plates (e.g. for 384 well plates 0.01 - 0.2 ng / well of sample are needed))